Qpcr Efficiency Calculator
Calculate qPCR amplification efficiency from the slope of the standard curve.
Formula
Efficiency = (10^(−1/slope) − 1) × 100%
- Perfect efficiency is 100% (slope = −3.32), meaning doubling every cycle.
- Acceptable range: 90–110%.
- Efficiency > 110% suggests inhibitors or non-specific amplification.
- Run at least 3 replicates per dilution point for reliable R².
Slope = −3.32
Inputs
- Standard Curve Slope: -3.32
E = (10^(1/3.32) − 1) × 100 ≈ 100% — perfect doubling efficiency.
Frequently asked questions
What slope corresponds to 100% efficiency?
A slope of −3.32 corresponds to exactly 100% efficiency (perfect doubling).
What R² value is acceptable?
R² ≥ 0.99 is ideal; ≥ 0.98 is acceptable for most applications.